Coding
BD-CIB1

Part:BBa_K1884001

Designed by: Chengrong Xie   Group: iGEM16_SZU-China   (2016-10-07)
Revision as of 11:46, 16 October 2016 by Nllenwalker (Talk | contribs)


Gal4BD-CIB1

Cryptochrome-interacting basic-helix-loop-helix 1(CIB1) is a protein-coded gene. The product of this gene expression is a basic helix-loop-helix (bHLH) protein which would interact with cryptochrome 2 (CRY2), a blue light stimulated photoreceptor, when exposed to blue light. This part is a Gal4 DNA binding domain fused to C terminus of CIB1.

Usage and Biology

This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA binding domian fused to its C terminus. In order to control DNA transcription by blue light, the system is based on a two-hybrid interaction in which a light-mediated protein brings together two halves of a split transcription factor. CRY2 will disconnected with CIB1 in the dark and halt the DNA transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137


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Categories
Parameters
None