Part:BBa_J45017:Design
isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 307
Illegal NgoMIV site found at 711
Illegal NgoMIV site found at 836
Illegal NgoMIV site found at 847
Illegal NgoMIV site found at 1128
Illegal NgoMIV site found at 1596 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Salicylate is made from chorismate via isochorismate. The reactions are catalysed by PchA (isochorismate synthase) and PchB (isochorismate-pyruvate lyase). Combined expression of the two proteins from one BioBrick part successfully generates salicylate in E. coli. For additional design features see [http://parts.mit.edu/wiki/index.php/MIT_2006 the MIT iGEM 2007 wiki].
Source
PchB and PchA are found in Pseudomonas aeruginosa. The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Reimmann from the Universite de Lausanne. Information about Dr. Reimmann's work is available at [http://www.unil.ch/dmf/page16594.html her website].
References
L. Serino, C. Reimmann, H. Baur, M. Beyeler, P. Visca & D. Haas. 1995. "Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa." Mol. Gen. Genet. 249, 217-228. This is the original paper describing pchBA.
C. Gaille, C. Reimmann & D. Haas. 2003. "Isochorismate synthase (PchA), the first and rate-limiting enzyme in salicylate biosynthesis of Pseudomonas aeruginosa." J. Biol. Chem. 278, 16893-16898. Information about the enzyme encoded by PchA.
J. Zaitseva, J. Lu, K.L. Olechoski & A.L. Lamb. 2006. "Two crystal structures of the isochorismate pyruvate lyase from Pseudomonas aeruginosa." J. Biol. Chem. 281,33441-9.