Device

Part:BBa_K1932007

Designed by: Jin Liu   Group: iGEM16_Jilin_China   (2016-10-14)
Revision as of 08:03, 16 October 2016 by JinLiu (Talk | contribs)


This device is constructed for the expression of TAT-apoptin fused with tmp1.

This device is constructed to express the fusion protein, TAT-apoptin and the region of tmp1 is included to increase the secretion quantity of the protein. Among the subparts, BBa_K1932000 is added as a promoter to regulate the expression of exogenous protein in Bifidobacterium with. BBa_K1932001 is included into the device to increase the stability of the device in Bifidobacterium. BBa_K1932003 encodes the signal peptide, tmp1, which can direct the export of the protein from Bididobacterium. BBa_K1932004 encodes TAT-apoptin, which acts as the effector protein to kill the cancer cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1059


[edit]
Categories
Parameters
None