Coding
pchBA

Part:BBa_J45017:Design

Designed by: MIT IGEM 2006   Group: iGEM06_MIT   (2006-08-21)
Revision as of 02:08, 20 July 2007 by Scmohr (Talk | contribs) (→‎References)


isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 711
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 847
    Illegal NgoMIV site found at 1128
    Illegal NgoMIV site found at 1596
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Salicylate is made from chorismate via isochorismate. The reactions are catalysed by PchA (isochorismate synthase) and PchB (isochorismate-pyruvate lyase). Combined expression of the two proteins from one BioBrick part successfully generates salicylate in E. coli.

Source

PchB and PchA are found in Pseudomonas aeruginosa. The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Reimmann from the Universite de Lausanne. Information about Dr. Reimmann's work is available at [http://www.unil.ch/dmf/page16594.html her website].

References

L. Serino, C. Reimmann, H. Baur, M. Beyeler, P. Visca & D. Haas. 1995. "Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa." Mol. Gen. Genet. 249, 217-228. This is the original paper describing pchBA.

C. Gaille, C. Reimmann & D. Haas. 2003. "Isochorismate synthase (PchA), the first and rate-limiting enzyme in salicylate biosynthesis of Pseudomonas aeruginosa." J. Biol. Chem. 278, 16893-16898. Information about the enzyme encoded by PchA.

J. Zaitseva, J. Lu, K.L. Olechoski & A.L. Lamb. 2006. "Two crystal structures of the isochorismate pyruvate lyase from Pseudomonas aeruginosa." J. Biol. Chem. 281,33441-9.