Regulatory

Part:BBa_K2040100:Experience

Designed by: Chilam Poon   Group: iGEM16_NYMU-Taipei   (2016-10-11)
Revision as of 07:42, 16 October 2016 by Chilam Poon (Talk | contribs)



Applications of BBa_K2040100

We used forward primer 5' ACGTCCTGCAGAATCATGCAGCGCTATGAG 3' & reverse primer 5' ATAAGCGGCCGCCATGATGGTCTAGGGAACG 3' to extract PMcl1 and its 5' untranslated region (99bp downstream the promoter) from genomic DNA of Metarhizium anisopliae. The whole length is 2772bp.

Figure1. Amplify PMcl1 from gDNA


















Then we digested the DNA fragment with NotI and PstI in order to insert it into the backbone. However, when we ran gel electrophoresis to check the digestion result, we found that there is still one PstI restriction site inside the PMcl1 whcih is the one we did not know when we designed.





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