Part:BBa_J45017

isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate

PchBA codes for proteins used to generate salicylic acid (the precursor to methyl salicylate or wintergreen scent) from the common metabolite, chorismate. The two genes are at the downstream end of the PchDCBA operon that Pseudomonas species use to encode enzymes that make pyochelin, a molecule that helps them acquire iron. This part doesn't make a fusion protein, even though the coding regions overlap; the RBS for PchA is located at the end of the coding region of PchB.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 307
  Illegal NgoMIV site found at 711
  Illegal NgoMIV site found at 836
  Illegal NgoMIV site found at 847
  Illegal NgoMIV site found at 1128
  Illegal NgoMIV site found at 1596
  
• 1000
  COMPATIBLE WITH RFC[1000]