Generator

Part:BBa_K2012013

Designed by: Wenqi Huang   Group: iGEM16_HZAU-China   (2016-10-06)
Revision as of 05:51, 16 October 2016 by Johnson Huang (Talk | contribs)


Strong CheZ generator with a fusion GFP reporter

This generator contain promotor J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domain.It can generate CheZ with a high ouIt is used to detect the relationship of CheZ under different promotor strength and its swimming ability.

We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately
the image are as following


Figure 1.CL1 control was observed under light field and fluorescence field

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Figure 2.E.coli with BBa_K2012013 was observed under light field and fluorescence field;</p>
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1362


[edit]
Categories
Parameters
None