![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2042014
Designed by: Toky RATOVOMANANA Group: iGEM16_Evry (2016-10-04)
Revision as of 22:30, 15 October 2016 by RATOVOMANANA (Talk | contribs)
RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)
This is the sequence of the PLA producing operon. This sequence is optimized for P.putida and is constituted of two gene propionate CoA transferase (Pct) and PHA synthase (PhaC). Pct contain a mutation that allows him to take lactate as the acceptor and convert it efficiently into lactyl-CoA. The PhaC gene contains 4 amino acid mutation which were found to polymerize monomer of lactyl-coA into PLA homopolymer. This gene is placed upstream in the sequence and close of the promoter because polymerization is the bottleneck of the PLA producing system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2386
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1073
Illegal NgoMIV site found at 1352
Illegal AgeI site found at 148
Illegal AgeI site found at 461 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2792
Illegal BsaI site found at 3263
[edit]
Categories
Parameters
//cds/biosynthesis
//chassis/prokaryote/pputida
//chassis/prokaryote/pputida
None |