![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2042013
Designed by: Delphine Dessaux Group: iGEM16_Evry (2016-09-03)
Revision as of 22:19, 15 October 2016 by RATOVOMANANA (Talk | contribs)
RBS +PhaC_resinovorans + RBS + Pct
This is the sequence of the operon constituted of PHA synthase 1 gene from Pseudomonas resinovorans and Propionate CoA transferase from Clostridum propionicum. The operon use lactate as substract, convert it into lactyl-coA and do it polymerisation into PLA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 100
Illegal BamHI site found at 2386
Illegal XhoI site found at 185
Illegal XhoI site found at 1043 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1073
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 809
Illegal BsaI site found at 2792
Illegal BsaI site found at 3263
[edit]
Categories
Parameters
//cds/biosynthesis
//chassis/prokaryote/pputida
//chassis/prokaryote/pputida
None |