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Translational_Unit
Part:BBa_K2042012
Designed by: Delphine Dessaux Group: iGEM16_Evry (2016-09-03)
Revision as of 21:49, 15 October 2016 by RATOVOMANANA (Talk | contribs)
RBS + PhaC_sp. MBEL 6-19
This is the sequence of Pseudomonas putida ribosom binding site followed by Pseudomonas sp. MBEL 6-19 PHA synthase 1 gene. The gene is codon optimized for P.putida and contain four mutation (E130D/S325T/S477G/Q481K) allowing to take the (D)-lactyl-CoA and produce the PLA homopolymer. It is used in the biobrick BBa_K2042014
BBa_K2042014
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1073
Illegal NgoMIV site found at 1352
Illegal AgeI site found at 148
Illegal AgeI site found at 461 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/biosynthesis
//chassis/prokaryote/pputida
//chassis/prokaryote/pputida
None |