Translational_Unit

Part:BBa_K2042011:Design

Designed by: Jonathan Tellechea   Group: iGEM16_Evry   (2016-09-02)
Revision as of 21:22, 15 October 2016 by RATOVOMANANA (Talk | contribs) (→‎Source)

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RBS +PhaC_resinovorans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
    Illegal XhoI site found at 185
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 809


Design Notes

We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.

Source

The RBS were synthesized from the genomic sequence of P.putida and the PhaC gene from Pseudomonas resinovorans.

References