Translational_Unit

Part:BBa_K2042011:Design

Designed by: Jonathan Tellechea   Group: iGEM16_Evry   (2016-09-02)
Revision as of 21:21, 15 October 2016 by RATOVOMANANA (Talk | contribs) (→‎Source)


RBS +PhaC_resinovorans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
    Illegal XhoI site found at 185
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 809


Design Notes

We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.

Source

The RBS were synthesized from the genomic sequence of P.putida,

References