Part:BBa_K1995014

this part can bind heavy metal ions

Based on BBa_K1420001 (merA), we added INP-N to the upstream of merA. Using INP-N, we anchored merA out of the outer membrane of E.coli in order to increase the reducing efficiency of mercuric ions.

Ice nucleation protein (INP)

To increase the reducing efficiency and detect the concentration of heavy metals, we used INP to anchor merA out of the outer membrane of E. coli.

Ice nucleation protein (INP) is an excretion surface protein. It is widely used for establishing germ surface display system. It contains an internal repeated domain (IRD) and an N-terminal anchoring domain (fig.1). Studies have shown that decrease the time of repetition of IRD can also get a better-off rate of displaying. So we decreased the time of repetition of IRD to reduce pressure of synthesis of E. coli.

Binding heavy metals

We detect the reducing efficiency of IA and other parts and get the following chart. (Fig. 2)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]