Regulatory

Part:BBa_K2116025:Experience

Designed by: Asli Azizoglu   Group: iGEM16_ETH_Zurich   (2016-10-14)
Revision as of 16:58, 15 October 2016 by Aasli (Talk | contribs)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

We generated this part along with others to characterise efficiency of repression by EsaR. It was tested on a medium-low copy plasmid (origin of replication p15A), along with EsaR expressed under the same promoter [Bba_J23118], on a medium copy plasmid (pBR322) or a low copy plasmid (pSC101). It was also tested with two copies of EsaR per (pBR322) plasmid.


Dose response graph.


Different amounts of esaR.



User Reviews

UNIQ1677e587967c3bbf-partinfo-00000000-QINU UNIQ1677e587967c3bbf-partinfo-00000001-QINU