Part:BBa_K1830002:Experience

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Applications of BBa_K1830002

BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the L.lactis cell. It should be fused to the N-terminal or C-terminal of the protein of interest.

iGEMers who want to use the SPusp45, they should choose the BBa K1654007 part, we have verified that BBa K1654007 is functional in protein secretion.

Summary

We characterized that the 27 amino acids long SPusp45 signal peptide part BBa K1654007, but not the BBa_K1830002 part is the right choice that can be used to secret proteins out of L. lactis.

User Reviews

UNIQ18183edb671f4433-partinfo-00000000-QINU

• TMMU-China-2016

BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the L.lactis cell. It should be fused to the N-terminal or C-terminal of the protein of interest. iGEMers who want to use the SPusp45, they should choose the BBa K1654007 part, we have verified that BBa K1654007 is functional in protein secretion.

UNIQ18183edb671f4433-partinfo-00000002-QINU