Coding
Part:BBa_K2012009
Designed by: Jun Li Group: iGEM16_HZAU-China (2016-09-14)
Revision as of 14:35, 15 October 2016 by Johnson Huang (Talk | contribs)
RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal
Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ. These parts are present in plasmid pSB1C3, but there is also a constitutive promoter(J23100-derived)
So, for example, the EcoRI/PstI region of part K2012009 reads:
[[----Biobrick5' XbaI J23100 XbaI K2012009 Biobrick 3'
gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctagasequence part...actagtagcggccgctgcag |----- .]]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1321
[edit]
Categories
Parameters
None |