Composite

Part:BBa_K2066016

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)
Revision as of 14:31, 15 October 2016 by Jlmaniaci (Talk | contribs)


Constitutive Lac Repressor on UNS Standard

In order to characterize the transfer function of a promoter with and without RiboJ, it is necessary to induce the activity of that promoter. plLacO-1 and pTac are both repressed by the lac repressor, which in turn is repressed by IPTG— hence IPTG will induce the activity of plLacO-1 and pTac.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None