Composite

Part:BBa_K1897015:Design

Designed by: Ang Shi Hui   Group: iGEM16_NUS_Singapore   (2016-10-10)
Revision as of 14:29, 15 October 2016 by Shihuiangle (Talk | contribs) (→‎Design Notes)


LuxR + LuxI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1852
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 50
    Illegal AgeI site found at 1065
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is one HA tag available for characterisation of the LuxR and LuxI proteins respectively produced via western blot.

Source

These parts are derived from Vibrio fischeri.

References