Coding

Part:BBa_K2012009

Designed by: Jun Li   Group: iGEM16_HZAU-China   (2016-09-14)
Revision as of 14:28, 15 October 2016 by Johnson Huang (Talk | contribs)


RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal

Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ. These parts are present in plasmid pSB1C3, but there is also a constitutive promoter(J23100-derived) So, for example, the EcoRI/PstI region of part K2012009 reads: [Biobrick5' XbaI J23100 XbaI K2012009 Biobrick 3' gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctagasequence part...actagtagcggccgctgcag]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1321


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