Part:BBa_K1949060
Prhl(NM)-rbs-gfp
We simulated our final genetic circuits and found that the circuits did not work, because Prhl strength was too weak. (see [http://2016.igem.org/Team:Tokyo_Tech our work in Tokyo_Tech 2016 wiki]). We therefore considered using the improved Prhl (BBa_K1529310,BBa_K1529310) established by Tokyo_Tech 2014, but we noticed that they were inappropriate for two reasons (see [http://2016.igem.org/Team:Tokyo_Tech our work in Tokyo_Tech 2016 wiki]). Then, we decided to improve Prhl Promoter and obtain our original improved Prhl (included in BBa_K1949060) that suited our goal.
Characterize and Improvement
Our purpose is to create strong Prhl for our final genetic circuits. This experiment consists of the three parts below.
I. Improved Prhl by Tokyo_Tech 2014 and characterize rhlR assay
II. Improvement of the native Prhl
III. Comparison of the improved Prhl by Tokyo_Tech2014 to our original improved Prhl
The past improved Prhl did not suit for our final circuits and we could create the improved Prhl appropriate to our final circuits.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 723
None |