Reporter
GG98

Part:BBa_K2145105

Designed by: Laura Xinyu Chen, Katie Maruna, Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-14)
Revision as of 04:20, 15 October 2016 by Lchen (Talk | contribs)


GFP/RFP reporter (Reverse/Reverse) with random 500bp spacer

This plasmid contains a GFP and an RFP reporter separated by a 500-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" (or a random sequence in) between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1661
    Illegal AgeI site found at 1773
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None