![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2172006
Designed by: Bowen Xiao Group: iGEM16_CIEI-BJ (2016-10-14)
-SmCPS1-
This part is a gene circuit that can be used to test whether it is being expressed or not. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin restriction enzyme cleavage site (for adding other segments) and a GFP detection unit. When transformed into bacteria, e.g. E. coli, it can be used to test whether the bacteria are expressing the part not.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1896
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 657
Illegal BamHI site found at 255
Illegal XhoI site found at 391
Illegal XhoI site found at 415
Illegal XhoI site found at 1708
Illegal XhoI site found at 1918 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 60
Illegal NgoMIV site found at 1477
Illegal NgoMIV site found at 2041 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |