Part:BBa_K1981005

lsrFG

A Protein complex involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle. LsrF is responsible for catalyzing the transfer of an acetyl moiety from 3-hydroxy-5-phosphonooxypentane-2,4-dione to CoA to form glycerone phosphate and acetyl-CoA. LsrG is responsible for catalyzing the conversion of (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione (P-DPD) to 3-hydroxy-5-phosphonooxypentane-2,4-dione (P-HPD). In the absence of AI-2, LsrFG is repressed by LsrR. It can be induced by AI-2, via releasing of the LsrR repressor. In the absence of glucose, LsrFG was induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

AI-2 “Consumer” was constructed by overexpressing the lsrFG gene.

This part is originated from Escherichia coli strain K12.

Characterization

The colony PCR verification result for lsrFG is shown in Figure 1.

Figure 1: Colony PCR Verification for lsrFG

Protein-Data Table

lsrF

Protein data table for BioBrick BBa_K1981005 automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 10: (underlined part encodes the protein)
ATGGCAGAT ... GAAAAACAGTAA
ORF from nucleotide position 1 to 873 (excluding stop-codon)

Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

1
101
201

MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPAT
NRPVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQ
IIKTYYVEKGFERIVAGCPVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ*

Sequence features: (with their position in the amino acid sequence, see the list of supported features)

None of the supported features appeared in the sequence

Amino acid composition:

Ala (A)	31 (10.7%)
Arg (R)	16 (5.5%)
Asn (N)	10 (3.4%)
Asp (D)	19 (6.5%)

Cys (C)	5 (1.7%)
Gln (Q)	14 (4.8%)
Glu (E)	15 (5.2%)
Gly (G)	23 (7.9%)

His (H)	6 (2.1%)
Ile (I)	20 (6.9%)
Leu (L)	20 (6.9%)
Lys (K)	15 (5.2%)

Met (M)	14 (4.8%)
Phe (F)	9 (3.1%)
Pro (P)	13 (4.5%)
Ser (S)	14 (4.8%)

Thr (T)	13 (4.5%)
Trp (W)	2 (0.7%)
Tyr (Y)	8 (2.7%)
Val (V)	24 (8.2%)

Amino acid counting

	Total number:	291
	Positively charged (Arg+Lys):	31 (10.7%)
	Negatively charged (Asp+Glu):	34 (11.7%)
	Aromatic (Phe+His+Try+Tyr):	25 (8.6%)

Biochemical parameters

	Atomic composition:	C₁₄₀₁H₂₂₄₄N₃₉₂O₄₁₉S₁₉
	Molecular mass [Da]:	31892.7
	Theoretical pI:	6.09
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	22920 / 23233 (all Cys red/ox)

Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges

Codon usage

	Organism:	E. coli	B. subtilis	S. cerevisiae	A. thaliana	P. patens	Mammals
	Codon quality (CAI):	excellent (0.84)	excellent (0.82)	acceptable (0.60)	good (0.66)	good (0.77)	good (0.65)

Alignments (obtained from PredictProtein.org)
There were no alignments for this protein in the data base. The BLAST search was initialized and should be ready in a few hours.

Predictions (obtained from PredictProtein.org)

Subcellular Localization (reliability in brackets)

	Archaea:	-
	Bacteria:	-
	Eukarya:	-

Gene Ontology (reliability in brackets)

	Molecular Function Ontology:	-
	Biological Process Ontology:	0006096 0. (NaN%)

Predicted features:

	Disulfid bridges:	-
	Transmembrane helices:	-

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lsrG