Coding

Part:BBa_K2165000:Design

Designed by: Roya Amini-Naieni   Group: iGEM16_Washington   (2016-10-14)
Revision as of 02:46, 15 October 2016 by Roygoli (Talk | contribs) (→‎Source)


Violacein C gene codon-optimized for S. Cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

The online codon-optimization tool offered by Integrated DNA Technologies (https://www.idtdna.com/CodonOpt) was used to codon-optimize this sequence for use in yeast. Combinations containing RFC 10 illegal restriction sites were actively avoided.


Source

​VioC​ ​is​ ​originally​ ​from​ ​the​ violacein​ ​metabolic pathway​ ​in​ ​Chromobacterium​ ​Violaceum​, a type of Proteobacteria. The original vioC sequence for the bacteria chassis was submitted by the Alberta team in 2013 as K1161003 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K1161003) and has been codon-optimized for use in yeast in this part.

References