Reporter
MelA

Part:BBa_K274001

Designed by: Shuna Gould   Group: iGEM09_Cambridge   (2009-08-03)
Revision as of 02:16, 15 October 2016 by Thiban (Talk | contribs)


melanin pigment

Produces dark brown pigment output. The gene (melA) codes for a tyrosinase which produces the pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site.

iGEM Concordia 2016 is currently characterizing this part in more efficient manner by improving the expression of melA through a point mutation. Refer to parts BBa_K2045004 and BBa_K2045005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1332
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1184
    Illegal BsaI.rc site found at 489


[edit]
Categories
//function/reporter/color
Parameters
None