Part:BBa_K1061013
P tight promoter
It is a response element containing 7 consecutive TetO elements and a minimal CMV promoter. When binding to tTA or rtTA, the expression of the downstream gene will be highly activated. Besides, it can achieve low constitutive expression(almost undectectable) when not binding to tTA or rtTA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Funtional experiment
We conducted the functional experiment by co-tranfected the plasmid contains tTA and the plasmid contains P tight into Bosc, one kind of HEK-293 cell lines.Noted that we did not use tTA advance due to time limit(we construct the plasmid contains tTA advance later), and we will try that later,for improvement of expression control.
The after adding dox, the expression of dox is almost fully suppressed. We also get the quantitive data by western blot, which will be submitted later.
quantitive analysis
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Functional Parameters
| n/a | P tight promoter |
References:
<p>[1]http://www.clontech.com/CN/Products/Inducible_Systems/Tetracycline-Inducible_Expression/Gen2?sitex=10022:22372:US| n/a | P tight promoter |