![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K2100008
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-10)
pENTR pPRE3
The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.
When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain. The DBD can then bind to the PREs on the promoter, recruiting transcriptional machinery and activating transcription.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 211
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 211
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 211
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 211
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 211
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
Illegal BsaI.rc site found at 225
[edit]
Categories
Parameters
//awards/part_collection/2016
None |