Coding

Part:BBa_K2127002

Designed by: Dawson Zeng   Group: iGEM16_IngenuityLab_Canada   (2016-10-14)
Revision as of 01:04, 15 October 2016 by Registry (Talk | contribs)

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CP47 His-tagged with Lac Promoter

CP47 His-tagged with Lac Promoter synthesized by IDT DNA Inc.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 473
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 597
    Illegal AgeI site found at 1194
    Illegal AgeI site found at 1398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918
    Illegal SapI site found at 1595


[edit]
Categories
//binding/metal
//cds/enzyme
//cds/membrane
//chassis/prokaryote/cyanobacterium
//plasmid/expression
Parameters
chassisSynechocystis spp. PCC 6803
familyPhotosystem II Reaction Complex
functionLight Harvesting, Oxygen Reaction Complex Stabilization
insertExpressable
ligandsHistine, Fe(II), Chlorine, Mangenase, Calcium (II), Chlorophyll, Beta-Carotene
protein47 kDa subunit
tagNickel Affinity Histine