Coding

Part:BBa_K2127001

Designed by: Nafaa Haddou   Group: iGEM16_IngenuityLab_Canada   (2016-10-14)
Revision as of 01:01, 15 October 2016 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Inducible High Expression His-Tagged Photosystem II CP47 subunit

TFBS plac LacO CP47his

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 658
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 782
    Illegal AgeI site found at 1379
    Illegal AgeI site found at 1583
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1103
    Illegal SapI site found at 1780


[edit]
Categories
//binding/metal
//cds/enzyme
//cds/membrane
//chassis/prokaryote/cyanobacterium
//plasmid/expression
Parameters
chassisSynechocystis spp. PCC 6803
familyPhotosystem II Reaction Complex
functionLight Harvesting, Oxygen Reaction Complex Stabilization
insertExpressable
ligandsHistine, Fe(II), Chlorine, Mangenase, Calcium (II), Chlorophyll, Beta-Carotene
positive_regulatorsPcpc560 Transcription Factor Binding Site
protein47 kDa subunit
tagNickel Affinity Histine