Reporter
Part:BBa_K1878001
Designed by: John Luke Garcia Group: iGEM16_Rice (2016-10-14)
iRFP713 (the fluorescent protein formerly known as "iRFP")
This fluorescent protein has an excitation wavelength of 690 nm and an emission wavelength of 713 nm. The extinction coefficient is 98000 M-1 cm-1. Its quantum yield is 6.3%. Its photostability half-life is 960 seconds. Its pKa is 4.5. Near-infrared fluorescent proteins are good for long-term in vivo imaging and exhibit high brightness in bacteria, mammalian cells, and mice.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 10
Illegal XhoI site found at 346
Illegal XhoI site found at 352 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 127
Illegal NgoMIV site found at 609 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |