Plasmid

Part:BBa_k2061002

Designed by: avi matityahu   Group: iGEM16_Tel-Hai   (2016-08-23)
Revision as of 21:29, 14 October 2016 by Almogang (Talk | contribs) (Created page with "This part contains 20 bp of target sequence in CFTR exon 11, PAM sequence (NGG) and the gRNA scaffold under the control of U6 promoter and terminator. The part was cloned in p...")

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This part contains 20 bp of target sequence in CFTR exon 11, PAM sequence (NGG) and the gRNA scaffold under the control of U6 promoter and terminator. The part was cloned in pSB1C3 between EcoRI and PstI sites. This part will serve for generating the first gRNA leading the CAS9 to the CFTR exon 11 for gene editing.

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