Device
Part:BBa_K2088014
Designed by: Shi Kejian Group: iGEM16_NAU-CHINA (2016-10-09)
J23101+RiboJ+M6+GFP+dT
The device contains a promoter(J23101), a riboJ, a regulating site(M6), a repoter(GFP) and a terminator(B0015). The regulating section M6 can bind the PPDA which could release the RBS to start translating, and the riboJ will stabilize the function of other parts. The GFP can be used as a repoter. In general J23101+riboJ+M6+GFP+B0015.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 881
[edit]
Categories
Parameters
None |