Composite

Part:BBa_K1937008

Designed by: Oumnia KARIM   Group: iGEM16_Toulouse_France   (2016-10-14)
Revision as of 17:31, 14 October 2016 by Oumniak (Talk | contribs)


AF-A antifungal operon in pSBBs0K-mini

(Chassis B. subtilis, carrier plasmid pSBBS0K) Length: 6558bp Background: AF-A is a part designed to be combined with AF-B to form an operon of 5 antifungal peptides. The interest of using several antifungals is to have a broad spectrum of fungi target, because each antifungal has its own characteristics.The peptides were designed to be expressed and secreted by Bacillus subtilis. This operon was also initially designed to be expressed under the control of the pNagA or pNagP promoter (BBa_K1937003 and BBa_K1937005 respectively). In spite of several cloning strategies, the AF-B construction was not obtained, likely due to the toxicity of one of the peptides for Bacillus subtilis. This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)

BBa K1937007-map1.png

BBa K1937008-genemap.png Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1228
    Illegal suffix found in sequence at 1256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1228
    Illegal NheI site found at 250
    Illegal NheI site found at 1250
    Illegal SpeI site found at 1257
    Illegal PstI site found at 1271
    Illegal NotI site found at 1234
    Illegal NotI site found at 1264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1228
    Illegal BglII site found at 970
    Illegal BglII site found at 5785
    Illegal BamHI site found at 1301
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1228
    Illegal suffix found in sequence at 1257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1228
    Illegal XbaI site found at 1243
    Illegal SpeI site found at 1257
    Illegal PstI site found at 1271
    Illegal NgoMIV site found at 2848
    Illegal AgeI site found at 5396
    Illegal AgeI site found at 6358
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 438
    Illegal BsaI.rc site found at 1026
    Illegal BsaI.rc site found at 2672
    Illegal BsaI.rc site found at 4111
    Illegal BsaI.rc site found at 6627
    Illegal SapI site found at 1589
    Illegal SapI.rc site found at 5609


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Categories
Parameters
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