Coding

Part:BBa_K1981003:Design

Designed by: Yulei Dang, Xinhao Song   Group: iGEM16_NKU_China   (2016-10-14)
Revision as of 17:20, 14 October 2016 by Songxinhao (Talk | contribs) (→‎Source)

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lsrC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 120


Design Notes

Point mutation was performed to mutate the restriction site of EcoRI at 752 bp and XbaI at 810 bp. Condon optimization was performed to meet themanufacture requirement of IDT to synthesize the DNA fragment.


Source

Sequence was acquired from genomic of Bacillus thuringiensis serovar thuringiensis str. IS5056. Part was chemically synthesized.

References