Coding
Part:BBa_K1985013
Designed by: Rita Adriani Group: iGEM16_Kent (2016-10-14)
Sequence coding for Sup35 with residues 1-61 with an arabinose inducible promoter
This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. This part contains three segments, the CsgA signal sequence, Sup35NM and a prion forming domain. Our improved BioBrick aims to optimize the aggregation process of amyloid fibrils with the addition of a short and specific prion forming domain of 61 aminoacid residues. This was inserted into the pSB1A3 backbone and uses the promoter BBa_K1321333.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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Categories
Parameters
None |