Coding

Part:BBa_K1985013

Designed by: Rita Adriani   Group: iGEM16_Kent   (2016-10-14)
Revision as of 16:12, 14 October 2016 by Registry (Talk | contribs)

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Sequence coding for Sup35 with residues 1-61 with an arabinose inducible promoter

This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. This part contains three segments, the CsgA signal sequence, Sup35NM and a prion forming domain. Our improved BioBrick aims to optimize the aggregation process of amyloid fibrils with the addition of a short and specific prion forming domain of 61 aminoacid residues. This was inserted into the pSB1A3 backbone and uses the promoter BBa_K1321333.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
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