Part:BBa_K1992003
Tar chemoreceptor tagged with GFP
Tar native RBS
Usage and Biology
usage and biology
GFP labeling is a very common way to examine the migration and location of certain proteins inside a living cell. here you can see such labeling in order to examine the migration of Tar chemoreceptor to the poles of E.coli bacteria.This property is critical for signal amplification and adaptation of the cell.
design
this part is a composite of 3 parts from the iGEM registry. the Tar receptor (K777000) and GFP(B0040) were taken were amplified from the kit and the flexible linker (J18921) was add by using reverse transcription PCR.
experiments and results
the fused Tar-GFP protein was cloned to our expression systems (K1992008,K1992009) in order to examine the migration of the receptor. By using a fluorescence microscope it can be seen in figure 1 that the receptor is closter in the poles of the bacteria as expected.
Figure 1
Fig1. (a) Tar-GFP expression in UU1250 strain cloned with K1992008 expression system. (b) Tar expression in UU1250 strain cloned with K1992004 expression system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1282
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2324
Illegal SapI.rc site found at 111
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