Part:BBa_K1965025

PPVp

Introduction

Plum pox virus protease (PPVp) is a protease from plum pox virus (PPV). PPVp is also known as nuclear inclusion a protein (NIa) that is one of the three viral proteases responsible for processing of viral polyprotein to functional segments (Adams2005, Garcia1991). PPVp is one of the most studied potyviral proteases after the TEVp. Its substrate (PPVs) has an aminoacid sequence NVVVHQ-A. In contrast to TEVp, it has been reported that PPVp is resistant to self-cleavage at the C-terminus (Zheng2008, Garcia1991).

Characterization

PPVp has well defined seven amino acid recognition motif PPVs which is defined by amino acid sequence NVVVHQ-A (Zheng2008, Uniprot). We included that sequence into the cyclic luciferase (cycLuc_PPVs) reporter construct.

This construct was expressed under CMV promoter. The coding sequence for PPVp was deposited in pSB1C3.

It was used as one of new orthogonal proteases in our protease based signaling platform. Figure 1. shows that PPVp successfully cleave its substrate and is orthogonal to other proteases (TEVp, SbMVp and SuMMVp).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 382
  Illegal BsaI.rc site found at 562