Composite

Part:BBa_I716009:Design

Designed by: Arthur Yu   Group: iGEM07_Berkeley_UC   (2007-06-28)
Revision as of 18:04, 9 July 2007 by Ayu (Talk | contribs)

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(cm)(rbs lib)(cytB5)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 475
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

composite part <cm><rbs><cytB5>
[digest] I716005 (pBca9145-cytB5)        (EcoRI, BglII; 2366+   9; L)
[digest] I716052 (pBca1101)              (EcoRI, BamHI; 2904+ 933; S)
[ligate]
[prod] I716009 (cm+rbs+cytB5)


Source

I716005 and I716052

References