Device

Part:BBa_K1919003

Designed by: Shiji Zhao   Group: iGEM16_SCU-China   (2016-09-07)
Revision as of 15:14, 14 October 2016 by Century (Talk | contribs)


T7 promotor (under the control of lacI) and downstream CecropinXJ

Biobrick BBa_K1919003 is a device, consisting of T7 promotor, RBS, TrxA tag, 6xHis tag, thrombin site, S tag, enterkinase site and the coding sequence of antimicrobial peptide CecropinXJ. Besides, T7 promotor is under the control of lacI protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2008
    Illegal BamHI site found at 2051
    Illegal XhoI site found at 2252
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1418
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/odor
Parameters
biologyBombyx mori
chassisE.coli
proteinLacI TrxA+6xHis tag+Thrombin site+S Tag+enterokinase site+CecropinXJ