Composite

Part:BBa_K1937007

Designed by: Oumnia KARIM   Group: iGEM16_Toulouse_France   (2016-10-14)
Revision as of 15:06, 14 October 2016 by Oumniak (Talk | contribs)


AF-A : operon of antifungal peptides

(Chassis E.coli, carrier plasmid pSB1C3, part destined for use in Bacillus subtilis) Length: 1006 bp Background: AF-A is a part designed to be combined with AF-B to form an operon of 5 antifungal peptides. The interest of using several antifungals is to have a broad spectrum of fungi target, because each antifungal has its own characteristics.The peptides were designed to be expressed and secreted by Bacillus subtilis. This operon was also initially designed to be expressed under the control of the pNagA or pNagP promoter (BBa_K1937003 and BBa_K1937005 respectively). In spite of several cloning strategies, the AF-B construction was not obtained, likely due to the toxicity of one of the peptides for Bacillus subtilis. This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)

BBa K1937007-map1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 250
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 438


[edit]
Categories
//collections/antimicrobial
Parameters
biology