Composite

Part:BBa_K2039002

Designed by: Coline Sivelle   Group: iGEM16_Paris_Saclay   (2016-10-13)
Revision as of 15:00, 14 October 2016 by Maxou67 (Talk | contribs)


Dimerization system FRB/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)

This part is the association of part K2039000 and K2039001.

The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria.

FKBP protein is linked to GFP 10 and FRB* protein is linked to GFP 11.

The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 716
    Illegal NheI site found at 739
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1255
    Illegal BamHI site found at 361
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 866
    Illegal AgeI site found at 1040
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None