Device
HSL report

Part:BBa_K1913014:Design

Designed by: Thomas Mathijs Swartjes   Group: iGEM16_Wageningen_UR   (2016-09-28)
Revision as of 14:58, 14 October 2016 by Tmswartjes (Talk | contribs) (→‎Design Notes)


3-oxo-hexanoyl-HSL GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 720


Design Notes

This part was made from elongating part BBa_K081012 with primers: 5' ggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaatactagatgcg 3' and VR (5' attaccgcctttgagtgagc 3), and then subsequently with primers: 5' gaattcgcggccgcttctagagacctgtaggatcgtacaggtttacgcaagaaaatgg 3' and VR (5' attaccgcctttgagtgagc 3). These primers were based on the BBa_R0062 sequence.

Source

Bacillus thuringiensis var. tenebrionis

References