Device

Part:BBa_K2088671

Designed by: Yuxin He   Group: iGEM16_NAU-CHINA   (2016-10-09)
Revision as of 14:53, 14 October 2016 by Summerice (Talk | contribs)

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J23100+B0034+mhbT+dT

This composite part includes a constitutive promoter (BBa_J23100), a RBS(BBa_B0034), a putative 3-hydroxybenzoate transporter gene(mhbT) and the double terminator (:BBa_B0015). The gene mhbT encodes a transport protein which is located on the cytomembrane. It can transport the 3-HBA into the cell.

This composite part can be used in degradation system of 3-hydroxybenzoate (3-HBA), and is also helpful in induction system whose derivant is 3-HBA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 227
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None