Coding

Part:BBa_K1981007

Designed by: Yulei Dang   Group: iGEM16_NKU_China   (2016-10-14)
Revision as of 14:43, 14 October 2016 by Songxinhao (Talk | contribs) (→‎Usage and Biology)


mtn

S-ribosylhomocysteine lyase. Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 475
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

The AI-2 “supplier” was constructed by overexpressing the luxS gene.

This part was originated from Bacillus amyloliquefaciens LL3.

Characterization

Protein-Data Table

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Categories
Parameters
None