Coding

Part:BBa_K2088668

Designed by: chenrui qin   Group: iGEM16_NAU-CHINA   (2016-10-08)
Revision as of 14:36, 14 October 2016 by Summerice (Talk | contribs)


B0030+pbaC

mhbM encodes 3-hydroxybenzoate 6-hydroxylase in Klebsiella pneumoniae M5a1, MhbM is an enzyme that utilizes 3-hydroxybenzoate (3-HBA) as substrate yielding gentisate. 3-HBA 6-hydroxylase introduces a new hydroxyl group at the para-position to the existing one and yields gentisate (2, 5-dihydroxybenzoate) , an intermediate step in the degradation of aromatic compounds in soil bacteria. Note that this part contains the native rbs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None