Intermediate

Part:BBa_K1913007

Designed by: Thomas Mathijs Swartjes   Group: iGEM16_Wageningen_UR   (2016-10-01)
Revision as of 14:04, 14 October 2016 by Tmswartjes (Talk | contribs) (→‎Usage and Biology)


434- and lambda cI operon for tuning protein balance

This part is intended to provide a balance between the 434- and lambda cI proteins depending on the strength of the RBS that is inserted upstream of 434 cI.


Usage and Biology

This part contains two viral genes: 434- and lambda cI (BBa_C0052 and part of BBa_K081007) expressed under the pBAD/AraC promoter (BBa_I0500). The 434 cI gene is not yet preceded by an RBS, allowing tuning of the cI protein balance. The 434 cI repressor protein encoded in this part should be more rapidly degraded (see design considerations) than the lambda cI protein. Therefore, changing the strength of the pBAD promoter in this part (with L-Arabinose or D-Glucose) should temporary shift the ratio between the two cI protein levels. We recommend using this part together with the modified lambda Prm promoter (BBa_I12006). Combining these parts should lead to interesting dynamics of expression under this modified lambda Prm promoter in response to changes in pBAD promoter strength.





Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1898
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None