Coding

Part:BBa_K1972009

Designed by: Shuli Liang   Group: iGEM16_SCUT-China_A   (2016-10-14)
Revision as of 13:57, 14 October 2016 by Shuli (Talk | contribs)


dszBCAD with Lac promoter

We constructed dszBCAD genes with lac promoter(a weaker promoter to aviod the generation of inclusion body) and choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.

Each single enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1266
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 488
    Illegal BamHI site found at 2070
    Illegal XhoI site found at 480
    Illegal XhoI site found at 3225
    Illegal XhoI site found at 4066
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 2514
    Illegal NgoMIV site found at 2729
    Illegal NgoMIV site found at 3033
    Illegal NgoMIV site found at 3153
    Illegal AgeI site found at 2688
    Illegal AgeI site found at 4254
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2220
    Illegal SapI.rc site found at 3537


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Parameters
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