Part:BBa_K1920006
pBAD-RBS-lysis-B0015
pBAD is an E.coli promoter that is induced by L-arabinose.In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription [1]. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription [1].[2] Using pBAD as the promoter of lysis allows us to control lysis process. However, under the enviroment of high concentration of glucose, pBAD will be inhibited[2]. Hence the this is a promoter can be controlled with two usual substance, glucose and arabinose. Yet, in order to make glucose effective to pBADthe concentration need to be higher than 0.01%.
[1]Schleif, R.Regulation of the l-arabinose operon of Escherichia coli. Trends in Genetics(2000) [2] I-Son Ng, et al., Simultaneous release of recombinant cellulases introduced by coexpressing colicin E7 lysis in Escherichia coli, Biotechnology and Bioprocess Engineering (2016)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |