Device
HSL report

Part:BBa_K1913014

Designed by: Thomas Mathijs Swartjes   Group: iGEM16_Wageningen_UR   (2016-09-28)
Revision as of 12:46, 14 October 2016 by Tmswartjes (Talk | contribs)


3-oxo-hexanoyl-HSL GFP reporter

This part reports (with GFP production) the presence of 3-oxo-hexanoyl-HSL, the autoinducer in the lux quorum sensing system from Vibrio fischeri.

Usage and Biology

3-oxo-hexanoyl-homoserine lactones (HSLs) are known to bind the 'lux-box' in the lux pR promoter (BBa_R0062). Binding of HSL in the promoter induces expression of downstream genes. HSLs function as signal molecules for inter bacterial communication: autoinducers. 3-oxo-hexanoyl-HSL specifically is the autoinducer in the lux quorum sensing system in Vibrio fischeri. This part consists of the lux pR promoter, an RBS


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 720


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Categories
Parameters
None