Composite

Part:BBa_K1918108:Design

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)
Revision as of 12:41, 14 October 2016 by SheepW (Talk | contribs) (→‎Source)

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CMV-Kozak-EBFP2N(1-154)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 756
    Illegal XbaI site found at 1236
    Illegal SpeI site found at 84
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 756
    Illegal SpeI site found at 84
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 756
    Illegal BamHI site found at 739
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 756
    Illegal XbaI site found at 1236
    Illegal SpeI site found at 84
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 756
    Illegal XbaI site found at 1236
    Illegal SpeI site found at 84
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 717


Design Notes

AIM: EBFP2N(1-154) could be expressed in cell all time; ELEMENTS: EBFP2N(1-154), CMV

Source

PCR from the existing plasmid in xie lab

References